SAMPLE & LIBRARY QC CHECKS

MGS will run client samples on the Qubit® Fluorometer or PerkinElmer Victor Plate Reader using the Quant-IT dsDNA HS Assay, as a quantification check before library preparation. The service will also run client and service prepared libraries on the Qubit® Fluorometer or PerkinElmer Victor Plate Reader, and the PerkinElmer LabChip® GX Touch HT instrument, as a quality control check before sequencing on the Illumina MiSeq™ instrument.

 

 Qubit® Fluorometer and PerkinElmer Victor Plate Reader

The following Invitrogen® assays are run:

• Quant-iT™ HS DNA Assay or Quant-iT BR DNA Assay – For genomics DNA and PCR amplicon samples, and MGS and client prepared libraries
• Quant-iT™ RNA Assay – For Total RNA and mRNA samples#

NOTE: # The Quant-iT™ HS DNA Assay is run to determine the percentage of DNA contamination for RNA samples. % contamination should be ≤ 10%.

 

PerkinElmer GX Touch HT Instrument

The following PerkinElmer LabChip® assays are run:

• High Sensitivity DNA Assay – MGS and client prepared libraries.

If the samples, client prepared libraries or service prepared libraries do not meet our quality or quantification standards, you will be required to sign an MGS disclaimer should you wish to continue with the processing of the samples and/or libraries.

Refer to "Submission of Work" for details.

 

Summary of QC Requirements

 

Customer Requirements	MGS Services QC Checks
Genomic, Metagenomic and PCR Amplicon samples# -       1-2% Agarose gel -       Spectrophotometer or Fluorometer to measure concentration and Spectrophotometer to measure OD260/280 and OD230/260 ratios	Genomic, Metagenomic and PCR Amplicon Sequencing samples -       Qubit® Fluorometer or PerkinElmer Victor Plate Reader
Total RNA and Small RNA samples# -       Bioanalyzer (if you have access) -       Formaldehyde 1% Agarose gel (if you do not have access to Bioanalyzer) -       Fluorometer to measure concentration and Spectrophotometer to measure OD260/280 and OD230/260 ratios	Total RNA and Small RNA samples -       Qubit® Fluorometer or PerkinElmer Victor Plate Reader
Libraries -       Service will accept prepared libraries from clients however an MGS disclaimer will be included in the Project Plan which the client needs to sign before proceeding with the Project	Libraries -       PerkinElmer GX Touch HT instrument using LabChip® DNA High Sensitivity Assay -       Qubit® Fluorometer or PerkinElmer Victor Plate Reader

NOTE: # MGS provide a LabChip Service. Refer to the MGS website for details.

 

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Last updated on Tuesday 10 October 2017